Agents assigned to this containment level include a broad spectrum of indigenous moderate risk agents for which vaccinations or therapies are generally available. They are transmissible to humans through accidental ingestion or entry through broken skin or contact with the mucous membranes.

• Hepatitis A, B, C, D
• Salmonella spp.
• Adenovirus
• Staphylococcus aureus

BSL-2 is required at a minimum for manipulating viable human derived materials and is recommended level for animal diagnostic laboratory operations.

The focus is on aerosol minimization and attention to sharps precautions. Aerosol-generating procedures are performed in a biosafety cabinet.