The primary approach is that of bacterial physiology, coupled with biochemical and genetic analysis. We measure
activities in the field and isolate organisms capable of such activities. Studies of these bacteria in the laboratory
allow us to develop more definitive methods for measuring activities in the field, including molecular methods for the
identification of organisms.
Examples of student research in the past few years have included: 1) mechanistic studies of metal reduction by
bacteria; 2) mechanisms of regulation in light emitting bacteria; 3) mechanisms of growth of bacteria on elemental
sulfur or nitrate as electron acceptors; and 4) anaerobic chemotaxis of bacteria. Current and ongoing work is aimed
at the study of the evolution of anaerobic respiratory pathways, studying several different pathways of respiration in
the organism Shewanella putrefaciens.
Forst S and KH Nealson. Molecular biology of the symbiotic-pathogenic bacteria Xenorhabdus spp. and
Photorhabdus spp. Microbiol. Rev. 60:21-43, 1996.
Krause B, Beveridge T, Remsen C, and Nealson KH. Structure and properties of novel inclusions in Shewanella
putrefaciens. FEMS Microbiol. Lett. 139-63-69, 1996.
Moser D and Nealson KH. Growth of Shewanella putrefaciens on elemental sulfur as an electron acceptor. Appl.
Environ. Microbiol. 62:2100-2105, 1996.
Kostka JE, Luther GW III, and Nealson KH. Chemical and biological reduction of Mn(III)-pyrophosphate complexes:
potential importance of dissolved Mn(III) as an environmental oxidant. Geochim. Cosmochim. Acta. 59:885-894,
1995.
Nealson KH, Moser D, and Saffarini DA. Anaerobic electron acceptor chemotaxis in Shewanella putrefaciens. Appl.
Environ. Microbiol. 61:1551-1554, 1995.
Kostka J and Nealson KH. Dissolution and reduction of magnetite by bacteria. Envir. Sci. Technol. 29:2535-2540,
1995.
Hosseini PK and Nealson KH. Symbiosis of luminous bacateria with soil nematodes; unsual regulation for an
unusual niche. Photochem. Photobiol. 62:633-640, 1995.
Tsapin AI, Burbaev DW, Nealson KH, and Keppen OI. Investigations of succinate dehydrogenase and fumarate
reductase in whole cells of Shewanella putrefaciens (MR-1 and MR-7) using electron spin resonance spectroscopy.
Appl. Magn. Reson. 9:509-516, 1995.