Department of Biological Sciences-UWM

University of Wisconsin - Milwaukee
Department of Biological Sciences

Electron Microscope Laboratory Equipment List
     
    Electron Microscopes
      Hitachi H-600 Transmission Electron Microscope (TEM)
        - operable in conventional, STEM, and SEM modes
        - capable of digital image acquisition (1024 x 1024 maximum resolution) in STEM and SEM modes
        - specimen holders: standard, dual, rotational (goniometer), low background (beryllium) current metering and cryotransfer (see below)
      Hitachi S-570 Scanning Electron Microscope (SEM)
        - operable in secondary electron (SE) and backscattered electron (BE) modes
        - capable of digital image acquisition (1024 x 1024 maximum resolution) in SE and BE modes

    Energy-dispersive X-ray Spectrometry (EDS)

      Both TEM and SEM are equipped with Oxford Link ISIS EDS systems
        - one atmosphere thin window SiLi detectors allow for the detection of elements Boron and higher
        - point and line scan capabilities, simultaneous acquisition of up to 30 digital dot maps

    TEM Specimen Preparation

      RMC MT 7000 Ultramicrotome (with cryoultramicrotomy attachment - see below)
      RMC MT 7 Ultramicrotome
      Reichert Ultracut E Ultramicrotome
      glass knife makers (LKB, RMC)
      LKB Multiplate

    SEM Specimen Preparation

      Critical point dryers (Sorvall, Balzers, Tousimis)
      Sputter coaters (SPI, CS Mini Coater)
      Vacuum evaporator (Edwards)

    Cryo Specimen Preparation

      RMC Propane Jet Freezer
      Leica AFS Freeze Substitution Apparatus
      RMC CR-21 attachment for RMC MT 7000 ultramicrotome
      Oxford CT 3500 Cryo Transfer System for TEM

    Light Microscopes

      Leica TCS SP2 Confocal System installed on a Leica upright compound microscope. Microscope equipped for DIC, phase contrast and epifluorescence microscopy.
        - 3 lasers provide 6 excitation lines for confocal imaging (458 nm, 476 nm, 488 nm, 514 nm, 543 nm and 633 nm)
        - 3 PMT channels for confocal acquisition, one transmitted light PMT
        - Spot RT color digital camera for image capture in other modes

      Olympus BH-2 upright compound microscope equipped for epifluorescence microscopy with a 100 watt mercury burner and 3 filter cubes (Olympus B L0910, B L0911 and B L012)

      Olympus IMT - 2 inverted compound microscope equipped with phase contrast optics.

      Olympus PM 10AD photomicrographic system for acquisition of 35 mm photographs with Olympus BH-2 and Olympus IMT-2 microscopes.

      Zeiss Large Universal upright microscope. Equipped for epifluorescence microscopy and 35 mm photography (Zeiss MC 63 camera system).

    Image Analysis, Storage and Output
      Fully equipped B&W darkroom (including condenser enlarger, point-source enlarger, automated print processor)
      Windows based image analysis computer and software (Photoshop 5.0, Optimas, VayTek VoxBlast)
      Iomega Zip drive
      Sony CD burner
      Agfa Duo Scan scanner
      Lexmark 1200 dpi laser printer
      Hewlett Packard color inkjet printer
      Xerox Tektronics 8200 Phaser color wax printer
      Polaroid 8000 Digital Palette film recorder

    Miscellaneous

      Wet lab for TEM and SEM specimen preparation including incubators, ovens, balances, stereo and light microscopes, centrifuges, microcentrifuge, pH meter, hot plates, stir plates, fume hoods, etc.

     

| UWM Biological Sciences |

Last Modified: July 2, 2003

Thomas P. Schuck