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Equipment available at the Biotech Core Facility:
(click
on the picture for a more detailed description)
Microarrays:
Microarrays are a leading edge method of
choice for large scale transcriptional
analysis essays and are one of
the well established
cornerstones of the facilty.
The facility includes all the necessary
equipment for the production, analysis and verification of Microarray Chip experiments. We are able to
produce our own microarray chips utilizing a Microgrid spotting robot
from Biorobotics that is able to print up to 100 slides per run. (seen
on the right)
 An automated slide
processing (ASP, GE Healthcare,
former Amersham Biosciences) unit
for hybridization, washing and drying of microarray slides for up to to
6 slides simultaneously is available in the facility. (seen on the left)
Utilization of this unit minimizes experimental variations between
experiments and chips which is essential for batch analysis of
microarray assays as all processes take place in a computer controlled
environment regarding temperature, sample flow rate, and exposure time.
For data acquisition of microarray slides we offer the use of a high
density laser
scanner (Axxon
Laboratories, Genepix 4200A) with a maximum resolution of 5nm per
pixel. The two lasers (532nm and
635nm) are continuously
adjustable during the scan process for real time channel
optimization.
The GenePix software controlling the scanner also allows for real time
data evaluation and can be configured for automated workflow
modes.
Purification
Purification
systems are a essential in modern biotechnological research. The
facility is equipped with modern
HPLC and FPLC chromatography systems and a Pulse-field-electrophoresis chamber.
Detection
The
facility is equipped with various detection system including a computer
controlled fluorescence
microscope with digital imaging, an automated plate reader,
spectrophotometers and a
fluorescence spectrum documentation system.
General
Equipment
A
lot of modern general equipment for molecular biological research is
available in the core facility
ranging from gel-documentation, over ultracentrifuges and
high-throughput PCR cyclers to
environmentally controlled incubators.
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